CSM Taq polymerases (Cold sensitive mutant Taq polymerases ) is a kind of HotStar Polymerase. Source from the mutant E. coli. CSM Taq polymerase unlike the monoclonal antibody based hot start Taq or chemical modified hot start Taq, Cold Taq is the cold sensitive mutant Taq. It retain the hot start ability throughout the whole amplification cycles.
Cold sensitive mutant Taq polymerases are designed for Hot Start PCR, it is not at low temperature. It offers excellent specificity and two-fold higher fidelity than wild-type Taq. It is designed for PCR with difficult templates such as GC-rich fragments and microsatellites.
Cold sensitive mutant Taq polymerases are particularly well suited to primer extension of Single Nucleotide Polymorphism (SNP) markers.
Cold sensitive mutant Taq polymerases maintain excellent specificity and minimal background even in conditions designed for high yield. In fact, even on genomic templates, the enzyme can be used with MgCl2+concentrations as high as 10 mM.
Cold sensitive mutant Taq polymerases are capable of extending through difficult regions, e. g. regions, which include inverted tandem repeats and those with high amounts of secondary structure.
Cold sensitive mutant Taq polymerases work in a totally unique way, involving improved nucleotide selection at the active site, and a much lower rate of mis-match extension, meaning that only perfectly aligned primers will be extended. As a result, the enzyme can give even higher specificity than hot-start (manual or automatic) techniques without the need for inconvenient pre-incubation steps.
Reaction Mixture Set Up
|Template||DNA <1 ug||as required|
|Forward Primer (10 μM)||2 μl||0.4 μM|
|Reverse Primer (10 μM)||5 μl||1x|
|2.5mM each dNTPmix||4 μl||200μM each|
|CSM Taq DNA polymerase, 5U/μl||0.4 μl||2 unit|
|ddH2O to final volume||50μl||Not applicable|
Concentration: 5 U/μl
Storage: Store at -20°C
|PC05||csm Taq DNA Polymerase||Cold sensitive mutant Taq DNA Polymerases, 5U/μl|