Manual

Product Number: PC11

Shipping and Storage

Ice pack transportation; Stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Components

Description

HS Taq DNA polymerase is a chemically modified Taq DNA polymerase, whose activity is completely blocked at room temperature and only released after heating at 95 ℃, which can prevent non-specific amplification and primer dimerization during sample preparation and reaction heating stages. Compared with antibody based hot start Taq enzymes, HS Taq DNA polymerase activity is more thoroughly blocked and has higher rigor; Compared with existing chemically modified hot start Taq enzymes, the activation time of HS Taq DNA polymerase is only 5 minutes, which is compatible with existing PCR programs. HS Taq DNA polymerase, combined with an optimized buffer system, can minimize non-specific amplification and primer dimers to the greatest extent possible, bringing extremely high sensitivity and specificity, making it very suitable for amplifying low copy genes from complex templates.

Application

Mainly used for DNA amplification reactions in animals, plants, and microorganisms. Hot start enzymes have 5 ‘-3’ exonuclease activity and can be used for fluorescence quantitative PCR reactions. The use of hot start amplification is a common method to improve PCR specificity, and hot start enzymes are a good choice. In addition, due to its high specificity and sensitivity, it is widely used in various fields such as constructing cDNA libraries, generating large amounts of DNA sequencing, mutant analysis and construction, gene isolation, genetic disease diagnosis, forensic identification, etc.

Unit definition

The activity of consuming 10 nmol of whole nucleotide as an acidic insoluble substance within 30 minutes at 74℃ using activated salmon sperm DNA as a template/primer is defined as one activity unit (U).