Manual

Product Number: PC06

Shipping and Storage

Ice pack transportation, stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Components

Description

Super Fidelity DNA Polymerase is a new generation of ultra fidelity DNA polymerase modified from Pfu DNA Polymerase, which has greatly improved its long segment amplification ability, amplification specificity, and amplification yield. By optimizing the reaction buffer and using simple templates such as lambda DNA and plasmids, fragments up to 40kb can be effectively amplified; Using complex templates such as genomic DNA, fragments up to 20kb can be amplified; The use of cDNA templates can effectively expand fragments up to 10kb in length. Its mismatch rate is 1/53 of that of ordinary Taq enzymes and 1/6 of that of Pfu enzymes, and the amplification speed can reach 15-30 seconds/kb. High fidelity and excellent amplification efficiency make Super Fidelity DNA Polymerase suitable for direct PCR of bacterial, fungal, plant tissue, animal tissue, or whole blood samples, with amplification products being flat ended.

Application

This product is suitable for PCR reactions using genomic DNA, cDNA, Plasmid DNA, and crude samples as templates.

1. High fidelity PCR and vector construction;
2. Gene cloning;
3. Gene directed mutagenesis;
4. High throughput PCR and sequencing.