Product Number: RE0569

Shipping and Storage

-20℃.

Components

Description

FlashCut™  Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases in the universal FlashCut™  Or FlashCut™  Color Buffer has excellent activity and can complete enzyme digestion within 5-15 minutes. In addition, our company dephosphorylation and ligation reagents are available on FlashCut™  Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™  Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis.  FlashCut™  The migration rate of red dye of Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is close to each other; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RCPB01

Shipping and Storage

1. -15°C below, protected from light, sealed.
2. Transportation stability: It is transported in an ice pack to keep it active.
3. Storage stability: Recombinant Carboxypepti-dase B can be stored for at least 24 months at – 15°C below. 25mM Tris-HCl (pH 8.0) was dissolved and stored at -15°C below, and repeatedly frozen and thawed 15 times without loss of activity. After dissolution, it is re-commended to keep it at 2~8 ℃ for not more than 10 days, and the enzyme activity keeps more than 95% for 5 days, and more than 90% for 10 days.

Description

Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, spermase, is a metalloproteinase that specifically hydrolyzes the protein’s C-terminal basic amino acid (lysine, arginine and histidine). The product is isolated and purified from recombinant Pichia pastoris, followed by multi-step isolation and purification, filtration and then freeze-dried. Its amino acid sequence is identical to that of natural porcine carboxypeptidase B. It possesses the same enzyme properties as those of natural carboxypeptidase B. This product has no exogenous virus contamination, does not contain enzyme inhibitors such as DFP, PMSF and TLCK, no miscellaneous enzymes such as chymotrypsin, no trypsin activity, and no other side reactions in the enzymatic reaction. This product belongs to GMP grade genetically engineered enzyme, residue HCP≤0.01%, residue DNA≤100ng/mg pro., bacterial endotoxin <100EU/mg, microbial limit≤100CFU/g.

Advantages

1. No animal origin: Recombinant production, no animal viruses, no pathogenic substances, no pollution by exogenous factors, high safety.
2. High purity, high specific activity and high enzymatic specificity, no other heteroenzyme residues.
3. Production scale above 1000L.
4. Compliance: This product is produced in GMP workshops, and the production equipment and production environment meet the requirements of relevant regulations and comply with GMP guidelines.
5. Complete quality documents: the quality management system of this product is certified by ISO 9001: 2015. Relevant regulatory support documents are available upon request.

Features

Enzyme activity definition: The amount of enzyme that catalyzes the hydrolysis of 1μmol of hippoyl-L-arginine for 1 min at 25℃ and pH 7.65 is 1U.

Application

1. Production of various proteins or polypeptides, such as insulin and analogs, cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.
2. Quality testing of antibodies.
3. Determination of the C-terminal amino acid of a protein.

Product Number: PCM105

Shipping and Storage

For long-term storage, store at −20°C and avoid repeated freeze-thaw cycles.

The product exhibits enhanced storage stability and can remain stable for up to 65 days at 37°C.

Components

Description

Real Stable qPCR Probe Master Mix (+UDG) is a probe-based qPCR master mix with excellent storage stability. The product remains stable for more than 98 days at 37°C, significantly improving its suitability for ambient-temperature transportation and non-cold-chain storage.

This 2× master mix contains the major components required for qPCR amplification. Only template DNA, primers, and probe need to be added before use.

The system incorporates an antibody-mediated hot-start Taq DNA polymerase together with an optimized qPCR buffer system, enabling efficient and highly specific real-time PCR amplification.

In addition, the master mix incorporates a dUTP/UDG carryover contamination prevention system. UDG degrades previously amplified PCR products containing dU before amplification, thereby reducing the risk of carryover contamination and false-positive results.

Features

1. Excellent lot-to-lot consistency
2. Excellent storage stability
3. Broad linear detection range
4. High PCR sensitivity
5. Reduced risk of false-positive results
6. Suitable for a wide range of qPCR applications

Product Number: PCK68

Shipping and Storage

For long-term storage, store at −20°C and avoid repeated freeze-thaw cycles.

The product exhibits enhanced storage stability and can remain stable for up to 65 days at 37°C.

Components

Description

Real Stable RT-qPCR Mix is a one-step RT-qPCR master mix designed for sensitive, efficient, and convenient RNA detection.

The system incorporates a genetically engineered, highly thermostable reverse transcriptase enzyme system with enhanced storage stability, reducing the dependence of conventional RNA detection reagents on strict cold-chain transportation and storage conditions. The product remains stable for up to 65 days at 37°C while maintaining excellent amplification efficiency and consistent fluorescence performance.

Its enhanced stability significantly improves flexibility during transportation and storage and makes the product particularly suitable for point-of-care testing (POCT) and large-scale, high-throughput automated testing applications. The system also incorporates a dUTP/UDG carryover contamination prevention system and RNase inhibitor to reduce the risk of false-positive results caused by carryover amplification products while helping maintain RNA template integrity.

Features

1. Excellent storage stability
2. Broad linear detection range
3. Excellent lot-to-lot consistency
4. Integrated dUTP/UDG carryover contamination prevention system
5. Contains RNase inhibitor to help maintain RNA template integrity

Product Number: EK0939

Shipping and Storage

-20±5℃. For frequent use, store at 2-8℃ and avoid repeated freeze-thaw cycles.

Components

Description

SuperFastStar Universal Probe Mixture (UNG) is a 2× qPCR premix suitable for probe-based fluorescence quantitative detection. It contains Taq DNA Polymerase, Uracil-N-Glycosylase, PCR Buffer, dNTPs, Mg2⁺, K⁺, enhancers, and stabilizers. The Taq DNA Polymerase is a double-antibody-captured hot-start enzyme with polymerase activity inhibition rates exceeding 95% at 55℃ or below, effectively reducing nonspecific amplification at low temperatures. The added Uracil-N-Glycosylase and dUTP contamination prevention system catalyzes dsDNA and ssDNA containing uracil, releasing free uracil to minimize cross-contamination of amplification products. The unique PCR buffer system significantly enhances qPCR amplification efficiency, enabling accurate detection across a dynamic range of up to 6 logarithmic levels.

This product is suitable for single and multiple amplifications, featuring high detection sensitivity and excellent specificity. It can detect templates as low as single copies, supporting direct amplification of oral swabs and low-concentration blood samples. With strong versatility, it is widely used in gene expression and virus detection. Additionally, the premixed solution contains extremely low glycerol content, allowing direct freeze-drying with lyophilization protectants.

Storage: Store immediately upon receipt at -20°C ± 5°C for at least 2 years. Shipped with gel ice packs.

Specification:

Package: 5000KU/Tube

Quality control: GMP grade products are manufactured and tested under a GMP-compliant management system.

Notes: CAUTION: Not intended for direct in vivo use.

Product Number: SAP01

Shipping and Storage

Store at -20℃. Shelf‑life: 2 years.

Components

Description

Shrimp Alkaline Phosphatase (Shrimp Alkaline Phosphatase, rSAP) catalyzes the dephosphorylation of 5′ and 3′ phosphate monoesters on DNA and RNA. This phosphatase is widely used in molecular biology research, such as removing phosphate groups from the termini of DNA and RNA for subsequent cloning and probe end labeling. In cloning, it can dephosphorylate linear vectors to prevent self-ligation. Shrimp alkaline phosphatase is completely and irreversibly inactivated by incubation at 65°C for 5 minutes, eliminating the need to remove heat-sensitive phosphatases after ligation or end labeling. Based on these properties, it serves as an excellent alternative to the traditional phosphatase—Calf Intestinal Phosphatase (CIP).

Application

1. Dephosphorylation of DNA and RNA
2. Preventing the self-ligation of cloning vectors
3. Prepare the 5´-end labeled template

Unit Definition

1 unit refers to the amount of enzyme required to hydrolyze 1 umol of p-Nitrophenyl Phosphate (pNPP) to pNP per minute at 37℃.

Product Number: RT03GF

Shipping and Storage

Store at -20℃. Shelf‑life: 2 years.

Components

Description

Reverse transcriptase initiates synthesis of complementary DNA strand using RNA as template. Thermostable M‑MLV Reverse Transcriptase (RNase H) is a mutant variant of M‑MLV reverse transcriptase. Multiple point‑mutations eliminate the RNase H active site, which greatly diminishes RNase H activity. This reduces RNA degradation during reverse‑transcription reactions, improves yield of first‑strand cDNA and facilitates full‑length cDNA acquisition. Meanwhile, its thermal stability is enhanced, with optimal reaction temperature at 50℃.

Source

E. coli strain harbouring the gene for mutant Moloney Murine Leukemia Virus (M‑MLV) reverse transcriptase.

Unit Definition

One unit (U) is defined as the quantity of enzyme required to catalyze incorporation of 1nmol dTTP within 10 min at 37℃, using Poly(A) as template and Oligo(dT) as primer.

Application

First-strand cDNA synthesis; RT-PCR.

Storage Buffer

 20mM Tris-HCl (pH 7.5), 200mM NaCl, 0.25mM EDTA, 0.01 % NP-40 (v/v), 2.5mM DTT, 50 % Glycerol (v/v).

Purity Specification

After incubation of 200 U enzyme with 1μg 16S, 23S rRNA at 37℃ for 1 h, no visible change of electrophoretic bands can be observed.

Reaction Buffer

5× RT Buffer: 250mM Tris‑HCl (pH 8.3), 15mM MgCl₂, 375mM KCl, 50mM DTT.

Product Number: LG02

Shipping and Storage

Ship with ice packs; store at −20℃. Valid for 2 years. Avoid repeated freeze‑thaw cycles.

Components

Description

T4 DNA Ligase catalyses the formation of phosphodiester bonds between adjacent 5’‑phosphate termini and 3’‑hydroxyl termini on double‑stranded DNA or RNA. This enzyme can catalyse ligation between blunt‑end or sticky‑end fragments; it can also repair nicks within double‑stranded DNA, RNA, or DNA/RNA hybrid duplexes.

Application

Ligation of DNA inserts with vector DNA; ligation of DNA fragments with linkers or adapter DNA.

Unit definition

One unit (U) is defined as the amount of enzyme required to ligate greater than 50 % of DNA fragments from λDNA‑Hind III digest in a 20μL ligation system containing 6 μg substrate, incubated at 16℃ for 30 min.

Product Number: TN5T02

Shipping and Storage

Ship on dry ice; store at −80℃. Valid for 6 months. Avoid repeated freeze‑thaw cycles. Store transposon DNA at −80℃, valid for 1 year.

Components

Description

Tn5 Transposase is a highly‑active engineered mutant Tn5 transposase derived from E. coli. It efficiently mediates random insertion of Tn5 transposons into target DNA sequences, exhibiting high transposition insertion efficiency against both eukaryotic and prokaryotic DNA. This product specifically recognizes DNA fragments flanked by Mosaic End (ME) sequences (including ME‑containing primers) to assemble Tn5 transposomes. The assembled transposomes bind randomly to target DNA, cleave the DNA, and insert their cargo DNA fragment. Tn5 transposase is widely applied in in‑vitro transgenesis (integration of exogenous genes into host cells) and Next‑Generation Sequencing (NGS) library construction.

Application

This product can be used for DNA fragmentation and adapter tagging during NGS library preparation; incorporation of sequencing primers into cloned DNA or plasmids; construction of bacterial gene‑knockout libraries; engineering of novel bacterial strains; insertional inactivation of target genes; insertion of T7 transcription promoters, antibiotic resistance markers and other elements into target DNA, etc.

Unit Definition

Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).

Specification

1. Source: Recombinant E. coli strain harbouring the Tn5 gene.
2. 5× Stop Buffer: 50mM EDTA (pH 8.0).