BSE TSE

Product Number: TE101

Shipping and Storage

Storage at -20±5℃.

Component

Description

This product is a protein cloned from the TelN Protelomerase gene expression of phage N15. It can specifically recognize the telRL sequence (56bp) on dsDNA, cleave dsDNA, and form a covalently closed end at the cleavage site, effectively transforming circular DNA into linear DNA with a closed end. The closed ended linear DNA generated by TelN Protelomerase treatment has stable performance, long half-life, and only introduces two 28 bp short sequences except for necessary sequences. It can encode long, complex, or unstable DNA sequences, does not contain bacterial sequences, and has a strong expression profile.

Application

This product can be used in fields such as DNA vaccine development, mRNA vaccine development, virus vector preparation, DNA data storage, etc.

Features

This product has strong specificity and can specifically cleave the recognized DNA sequence (telRL) to form a covalently closed end. The recognition sequence is as follows:

Unit definition

1 unit refers to the amount of enzyme required to cleave 0.5μg BsaI linearized plasmid (313fmol telRL recognition site) in a 50μL 1 × TelN Reaction Buffer reaction buffer system at 30℃ for 30 minutes.

BSE TSE

Product Number: RPJ06

Shipping and Storage

Storage at -15℃ and below, away from light, sealed.

Description

Trypsin is a serine protease that selectively hydrolyzes peptide chains composed of the carboxyl terminus of lysine or arginine in proteins. This product is a recombinant trypsin powder isolated and purified from recombinant Pichia pastoris, and then freeze-dried.

This product belongs to the genetic engineering enzyme of biochemical grade and does not contain enzyme inhibitors such as DFP, PMSF, and TLCK. It has the same activity and specificity as natural trypsin. The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, as well as metal ion chelating agents such as EDTA.

Application

1. Used for the production of biopharmaceuticals, such as insulin and GLP-1 drugs.
2. Research on protein structure and function, such as protein mass spectrometry, sequencing, and peptide mapping analysis.

Product advantages

1. No animal origin: no animal viruses, no pathogenic substances, no external factor contamination, high safety.
2. High purity: high specific activity; The residual host protein is less than the limit requirement for biological products.
3. Complies with the standards of the Chinese Pharmacopoeia 2020 edition and USP43.
4. Production scale of over 1000L.
5. Compliance: The production equipment and environment comply with relevant regulatory requirements and GMP guidelines.
6. Complete quality documents: Relevant regulatory support documents can be provided according to customer requirements.

BSE TSE

Product Number: RA03

Description

Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.

RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.

A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.

• CAS No.: 9001-99-4
• E.C.: 3.1.27.5
• Source: Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
• Activity: ≥40 Kunitz units/mg Protein
• Molecular weight: 14.3 kDa
• Form: Lyophilized Powder
• Grade: Molecular biology level
• Purity: ≥ 90%; (SDS-PAGE)
• Endotoxin: None detected (Tachypleus Amebocyte Lysate)
• Foreign activity: Endonuclease and exonuclease, none detected
• NICKase and DNase, none detected
• Optimal pH: 7.6 (activity range of 6-10)
• Optimal temperature: 60°C (activity range of 15-70°C)
• Shelf life: 3 years (-20°C)

Transportation and Storage

Storage temperature: -20°C.

Order

Stability Report

BSE TSE

Product Number: RA02

Description

Our Ribonuclease A Solution is a non-specific ribonuclease derived from bovine pancreas that has been modified through protein engineering technology. It is obtained through yeast expression and purification. It does not contain bacterial endotoxins expressed by prokaryotes. Functionally, RNase A is a ribonuclease that can hydrolyze the phosphodiester bond between the 5 ‘- ribose on a nucleoside and the phosphate group on the adjacent pyrimidine nucleoside 3’ – ribose. The resulting 2 ‘, 3’ – cyclic phosphate can be hydrolyzed into the corresponding 3 ‘- nucleoside phosphate. Therefore, single stranded RNA can be specifically degraded at the positions of C and U nucleotide residues. RNase A is very stable. RNase A exhibits high efficacy when acting on single stranded RNA.

This product is widely used to remove RNA contamination from DNA or protein samples.

This product is free from contamination by endonucleases and exonucleases, as well as protease contamination.

Quality Index

Note

For your safety and health, please wear lab clothes and protective gloves when using.

Product Number: TN5T02

Shipping and Storage

Store at -30 ~ -15℃. Transportation conditions: ≤ 0℃.

Components

Enzyme storage solution: 50mM HEPES (pH7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol.

Reaction Buffer (5×): 50mM HEPES (pH7.2), 500mM NaCl, 50mM MgCl₂.

Stop Buffer (5×): 50mM EDTA (pH8.0).

Description

Tn5 Transposase, It is a highly active Tn5 transposase mutant derived from E. coli, which can efficiently insert Tn5 transposons randomly into target sequences. Tn5 Transposase can specifically recognize DNA fragments containing chimeric end sequences (ME) at both ends (including primers containing ME sequences), ultimately forming Tn5 Transposomes, which can randomly bind to target DNA and cleave and insert the DNA fragments they carry. Tn5 transposase is widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and next-generation sequencing (NGS) library construction.

Application

This product can be used for fragmentation and adapter addition during the construction of next-generation sequencing (NGS) libraries; Introducing sequencing primers into cloned DNA or plasmids; Establishment of bacterial gene knockout library; Engineering transformation of new bacterial strains; Insertion inactivation of target genes; Insert T7 transcription promoter, resistance markers, etc. into target DNA, etc.

Unit Definition

Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).

Product Number: TN5T01

Shipping and Storage

Store at -80℃. Transportation conditions: ≤ 0℃.

Components

Enzyme storage solution: 50mM HEPES (pH7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol.

Reaction Buffer (5×): 50mM HEPES (pH7.2), 500mM NaCl, 50mM MgCl₂.

Stop Buffer (5×): 50mM EDTA (pH8.0).

Description

PA-Tn5 Transposase， It is a novel fusion enzyme (pA-Tn5 Transposase) that combines Protein A with a modified ultra-high activity Tn5 transposase to form a dual activity enzyme. It can efficiently insert Tn5 transposons randomly into target sequences. PA-Tn5 Transposase can specifically recognize DNA fragments containing chimeric end sequences (ME) at both ends (including primers containing ME sequences), ultimately forming Tn5 Transposomes, which can randomly bind to target DNA and cleave and insert DNA fragments carried by it. Tn5 transposase is widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and next-generation sequencing (NGS) library construction.

Application

This product can be used for fragmentation and adapter addition during the construction of next-generation sequencing (NGS) libraries; Introducing sequencing primers into cloned DNA or plasmids; Establishment of bacterial gene knockout library; Engineering transformation of new bacterial strains; Insertion inactivation of target genes; Insert T7 transcription promoter, resistance markers, etc. into target DNA, etc.

Unit Definition

PA-Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).

Product Number: PCK55

Shipping and Storage

Store at -20°C, with a shelf life of 12 months.

Components

Description

This product is a dedicated kit for one-step reverse transcription real-time fluorescence quantitative detection using the probe method. With extracted RNA as the template, reverse transcription and fluorescence quantitative detection are performed sequentially in the same reaction tube, ensuring simple operation while effectively preventing contamination and reducing pipetting errors. Based on high-efficiency reverse transcriptase, hot-start polymerase, and an optimized buffer system, it delivers excellent amplification performance for RNA templates with complex secondary structures and high GC content, making it highly suitable for detecting trace target genes such as RNA viruses. When using this product, simply add the template, primers, probes, ROX Reference Dye (used to correct fluorescence signal variations between wells, select based on the specific real-time PCR instrument), and water,

Adjust the working concentration to 1× to proceed with the reaction. It offers advantages such as rapid and simple operation, high sensitivity, strong specificity, and excellent stability, effectively minimizing human errors, saving PCR experimental time, and reducing contamination risks.

Application

Suitable for high-copy and low-copy gene detection; applicable to RNA templates with high GC content or complex secondary structures.

Product Number: PCK59

Shipping and Storage

Store at -20°C, with a shelf life of 12 months.

Components

Description

This kit is a specialized reagent for Real Time PCR using SYBR Green I intercalating fluorescence. When used for Real Time qRT-PCR, it enables continuous amplification within the same reaction tube without the need to open the tube for reagent addition, allowing real-time detection of amplified products. This eliminates contamination while improving detection sensitivity and experimental efficiency, making it highly suitable for detecting trace RNA, especially RNA viruses.  The kit includes an optimized OneStep Enzyme Mix (containing mutated TRUEscript M-MuLV H Minus reverse transcriptase, hot-start HotMaster Taq DNA polymerase, and RNasin inhibitor mix), as well as a unique 2×qRT-PCR SybrGreen Mix system suitable for reverse transcription and fluorescent PCR amplification. The M-MuLV (RNase H⁻) enzyme lacks RNase H activity, offering stronger extension capability and stability compared to M-MuLV. Additionally, this enzyme enhances heat resistance, enabling reverse transcription at 42-50°C and improving reverse transcription efficiency for templates with complex secondary structures or high GC content.  The inclusion of high-quality hot-start enzyme HotMaster Taq DNA Polymerase minimizes nonspecific amplification products throughout the PCR process, significantly improving the accuracy of fluorescent quantitative PCR. This product yields excellent standard curves across a wide quantitative range, enabling precise quantification of target genes with good repeatability and high reliability.

Application

Suitable for high copy and low copy gene testing; RNA templates with high GC content or complex secondary structures.

Unit definition

The activity of consuming 10 nmol of whole nucleotide as an acidic insoluble substance within 30 minutes at 74℃ using activated salmon sperm DNA as a template/primer is defined as one activity unit (U).

Product Number: RE0528

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0510

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Cannot be thermally deactivated, please use phenol chloroform extraction or column purification.