Product Number: TN5T02

Shipping and Storage

Store at -30 ~ -15℃. Transportation conditions: ≤ 0℃.

Components

Enzyme storage solution: 50mM HEPES (pH7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol.

Reaction Buffer (5×): 50mM HEPES (pH7.2), 500mM NaCl, 50mM MgCl₂.

Stop Buffer (5×): 50mM EDTA (pH8.0).

Description

Tn5 Transposase, It is a highly active Tn5 transposase mutant derived from E. coli, which can efficiently insert Tn5 transposons randomly into target sequences. Tn5 Transposase can specifically recognize DNA fragments containing chimeric end sequences (ME) at both ends (including primers containing ME sequences), ultimately forming Tn5 Transposomes, which can randomly bind to target DNA and cleave and insert the DNA fragments they carry. Tn5 transposase is widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and next-generation sequencing (NGS) library construction.

Application

This product can be used for fragmentation and adapter addition during the construction of next-generation sequencing (NGS) libraries; Introducing sequencing primers into cloned DNA or plasmids; Establishment of bacterial gene knockout library; Engineering transformation of new bacterial strains; Insertion inactivation of target genes; Insert T7 transcription promoter, resistance markers, etc. into target DNA, etc.

Unit Definition

Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).

Product Number: TN5T01

Shipping and Storage

Store at -80℃. Transportation conditions: ≤ 0℃.

Components

Enzyme storage solution: 50mM HEPES (pH7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol.

Reaction Buffer (5×): 50mM HEPES (pH7.2), 500mM NaCl, 50mM MgCl₂.

Stop Buffer (5×): 50mM EDTA (pH8.0).

Description

PA-Tn5 Transposase， It is a novel fusion enzyme (pA-Tn5 Transposase) that combines Protein A with a modified ultra-high activity Tn5 transposase to form a dual activity enzyme. It can efficiently insert Tn5 transposons randomly into target sequences. PA-Tn5 Transposase can specifically recognize DNA fragments containing chimeric end sequences (ME) at both ends (including primers containing ME sequences), ultimately forming Tn5 Transposomes, which can randomly bind to target DNA and cleave and insert DNA fragments carried by it. Tn5 transposase is widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and next-generation sequencing (NGS) library construction.

Application

This product can be used for fragmentation and adapter addition during the construction of next-generation sequencing (NGS) libraries; Introducing sequencing primers into cloned DNA or plasmids; Establishment of bacterial gene knockout library; Engineering transformation of new bacterial strains; Insertion inactivation of target genes; Insert T7 transcription promoter, resistance markers, etc. into target DNA, etc.

Unit Definition

PA-Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).

Product Number: PCK55

Shipping and Storage

Store at -20°C, with a shelf life of 12 months.

Components

Description

This product is a dedicated kit for one-step reverse transcription real-time fluorescence quantitative detection using the probe method. With extracted RNA as the template, reverse transcription and fluorescence quantitative detection are performed sequentially in the same reaction tube, ensuring simple operation while effectively preventing contamination and reducing pipetting errors. Based on high-efficiency reverse transcriptase, hot-start polymerase, and an optimized buffer system, it delivers excellent amplification performance for RNA templates with complex secondary structures and high GC content, making it highly suitable for detecting trace target genes such as RNA viruses. When using this product, simply add the template, primers, probes, ROX Reference Dye (used to correct fluorescence signal variations between wells, select based on the specific real-time PCR instrument), and water,

Adjust the working concentration to 1× to proceed with the reaction. It offers advantages such as rapid and simple operation, high sensitivity, strong specificity, and excellent stability, effectively minimizing human errors, saving PCR experimental time, and reducing contamination risks.

Application

Suitable for high-copy and low-copy gene detection; applicable to RNA templates with high GC content or complex secondary structures.

Product Number: PCK59

Shipping and Storage

Store at -20°C, with a shelf life of 12 months.

Components

Description

This kit is a specialized reagent for Real Time PCR using SYBR Green I intercalating fluorescence. When used for Real Time qRT-PCR, it enables continuous amplification within the same reaction tube without the need to open the tube for reagent addition, allowing real-time detection of amplified products. This eliminates contamination while improving detection sensitivity and experimental efficiency, making it highly suitable for detecting trace RNA, especially RNA viruses.  The kit includes an optimized OneStep Enzyme Mix (containing mutated TRUEscript M-MuLV H Minus reverse transcriptase, hot-start HotMaster Taq DNA polymerase, and RNasin inhibitor mix), as well as a unique 2×qRT-PCR SybrGreen Mix system suitable for reverse transcription and fluorescent PCR amplification. The M-MuLV (RNase H⁻) enzyme lacks RNase H activity, offering stronger extension capability and stability compared to M-MuLV. Additionally, this enzyme enhances heat resistance, enabling reverse transcription at 42-50°C and improving reverse transcription efficiency for templates with complex secondary structures or high GC content.  The inclusion of high-quality hot-start enzyme HotMaster Taq DNA Polymerase minimizes nonspecific amplification products throughout the PCR process, significantly improving the accuracy of fluorescent quantitative PCR. This product yields excellent standard curves across a wide quantitative range, enabling precise quantification of target genes with good repeatability and high reliability.

Application

Suitable for high copy and low copy gene testing; RNA templates with high GC content or complex secondary structures.

Unit definition

The activity of consuming 10 nmol of whole nucleotide as an acidic insoluble substance within 30 minutes at 74℃ using activated salmon sperm DNA as a template/primer is defined as one activity unit (U).

Product Number: RE0528

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.

Product Number: RE0510

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Cannot be thermally deactivated, please use phenol chloroform extraction or column purification.

Product Number: BS03

Shipping and Storage

Store at -20°C.

Components

Description

WellStart III BST DNA polymerase is a hybrid enzyme containing WarmStafi Bst DNA polymerase and a thermostable reverse transcriptase. WarmStafi Bst III features modifications over Bst 3.0, eliminating nonspecific amplification during room-temperature reaction setup and eliminating the need for a separate activation step, thereby enhancing reaction specificity. The thermostable reverse transcriptase is a genetically engineered novel enzyme with significantly improved cDNA synthesis speed and thermal stability, capable of tolerating reaction temperatures up to 60°C, making it suitable for reverse transcription reactions with RNA templates possessing complex secondary structures. WellStart III BST DNA polymerase can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT-LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80 ℃ for 5 minutes to inactivate.

Product Number: PC11

Shipping and Storage

Ice pack transportation; Stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Components

Description

HS Taq DNA polymerase is a chemically modified Taq DNA polymerase, whose activity is completely blocked at room temperature and only released after heating at 95 ℃, which can prevent non-specific amplification and primer dimerization during sample preparation and reaction heating stages. Compared with antibody based hot start Taq enzymes, HS Taq DNA polymerase activity is more thoroughly blocked and has higher rigor; Compared with existing chemically modified hot start Taq enzymes, the activation time of HS Taq DNA polymerase is only 5 minutes, which is compatible with existing PCR programs. HS Taq DNA polymerase, combined with an optimized buffer system, can minimize non-specific amplification and primer dimers to the greatest extent possible, bringing extremely high sensitivity and specificity, making it very suitable for amplifying low copy genes from complex templates.

Application

Mainly used for DNA amplification reactions in animals, plants, and microorganisms. Hot start enzymes have 5 ‘-3’ exonuclease activity and can be used for fluorescence quantitative PCR reactions. The use of hot start amplification is a common method to improve PCR specificity, and hot start enzymes are a good choice. In addition, due to its high specificity and sensitivity, it is widely used in various fields such as constructing cDNA libraries, generating large amounts of DNA sequencing, mutant analysis and construction, gene isolation, genetic disease diagnosis, forensic identification, etc.

Unit definition

The activity of consuming 10 nmol of whole nucleotide as an acidic insoluble substance within 30 minutes at 74℃ using activated salmon sperm DNA as a template/primer is defined as one activity unit (U).

Product Number: PC06

Shipping and Storage

Ice pack transportation, stored at -20℃, with a shelf life of 2 years, to avoid repeated freezing and thawing.

Components

Description

Super Fidelity DNA Polymerase is a new generation of ultra fidelity DNA polymerase modified from Pfu DNA Polymerase, which has greatly improved its long segment amplification ability, amplification specificity, and amplification yield. By optimizing the reaction buffer and using simple templates such as lambda DNA and plasmids, fragments up to 40kb can be effectively amplified; Using complex templates such as genomic DNA, fragments up to 20kb can be amplified; The use of cDNA templates can effectively expand fragments up to 10kb in length. Its mismatch rate is 1/53 of that of ordinary Taq enzymes and 1/6 of that of Pfu enzymes, and the amplification speed can reach 15-30 seconds/kb. High fidelity and excellent amplification efficiency make Super Fidelity DNA Polymerase suitable for direct PCR of bacterial, fungal, plant tissue, animal tissue, or whole blood samples, with amplification products being flat ended.

Application

This product is suitable for PCR reactions using genomic DNA, cDNA, Plasmid DNA, and crude samples as templates.

1. High fidelity PCR and vector construction;
2. Gene cloning;
3. Gene directed mutagenesis;
4. High throughput PCR and sequencing.

Manual

Product Number: RA01

Shipping and Storage

Sealed, dry.

Description

RNase A is an endonuclease that has been well studied and widely used. RNase A hydrolyzes RNA.

Application

1. Drugs: change host cell metabolism, inhibit virus synthesis,
2. Biochemical experiments: Catalyzed RNA degradation, which can specifically hydrolyze the RNA phosphate diester bond hybridized to THE DNA chain, so it can decompose the RNA chain in the RNA/DNA hybrid system.
3. Industrial production，decompose RNA and purify the product.

Specification

1. Source: Bovine (Porcine) pancreas.
2. Appearance: White or pale yellow lyophilized powder.
3. Solvent Transparentness: Not more opalescent than ref.suspensionⅡ.
4. Loss on drying: No more than 5.0%.
5. DNA: Not detectable.
6. Microbial limits: Pseudomonas aeruginosa: Negative; Salmonella: Negative; Staphylococcus aureus: Negative
7. Assay: No less than 50Kunitz units/mg
8. Protein (UV): No less than 65%

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