Manual

Product Number: PC09

Shipping and Storage

-20°C.

Components

Note: The 5×Golden Star Taq PCR Buffer of this product contains 8.5mM magnesium ions.

Description

Golden Star Taq DNA Polymerase is a chemically modified new and efficient Taq DNA Polymerase, which is completely blocked at room temperature, making the enzyme inactiveat low or normal temperature. Thus, non-specific amplification caused by non-specific binding of primers and templates or primer dimers can be effectively avoided at room temperature ,the activation of the enzyme must be incubated at 95°C for 10 minutes.The unique buffer system enables the enzyme to be widely used, and makes efficient amplification of templates with high GC content, complex secondary structure and low copy. Using this product for PCR amplification, the 3' end of the PCR product with an "A" base, can be directly used for T/A cloning.This product has strong specificity and can be directly used for downstream cloning or chip hybridization experiments without the need for glue recovery to remove the heteroband after PCR amplification. It is mainly for conventional PCR, RT-PCR, real-time PCR, multiplex PCR, gene chip analysis and SNP detection, especially for PCR reaction with high specificity requirements.

Activity Definition

Using activated salmon sperm DNA as template/primer, the amount of enzyme required to incorporate 10nmol deoxynucleotides into acidic insoluble material was defined as 1 active unit(U) at 74°C for 30 min.

Quality Control

The purity of SDS-PAGE was greater than 99%. No exogenous nuclease activity was detected. No host residual DNA was detected by PCR. It can effectively amplify single copy genes in the human genome. There was no obvious activity change after one month storage at room temperature

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