LA Taq DNA Polymerase three times higher than Taq DNA Polymerase
Long PCR Polymerase which synergistically generate long PCR products with greater yield and fidelity than Taq DNA Polymerase alone.
The fidelity of PCR is three times higher than with Taq DNA Polymerase.
The LA Taq is optimized for generation of very long amplicons: up to 40 kb with viral DNA and up to 15 kb with genomic DNA templates. The specially formulated LA Taq Buffer protects DNA from depurination and nicking during long thermal cycling.
The PCR products generated with the LA Taq DNA Polymerase are mostly 3′-dA tailed, which can be cloned in TA vector.
Functionally tested in generation of 40 kb amplicon with lambda DNA as template.
Concentration: 5 u/μl
Storage Buffer: The enzyme is supplied in: 20mM Tris-HCl (pH 8.0); 0.1mM EDTA; 1mM DTT; 100mM KCl; Stabilizers; 50% glycerol.
Storage: Store at -20°C.
Reaction PCR Mixture Set Up and Recommended thermal cycling conditions: The flowing is only an example which take a 20KB human genomic DNA as the template, that only for a reference, It can be adjusted according the length and the sequence of the template and the primer.
|Template||DNA <1 ug||as required|
|Forward Primer (10 μM)||1 μl||0.2-0.4 μM each|
|Reverse Primer (10 μM)||1 μl||0.2-0.4 μM each|
|10x LA Taq Buffer||5 μl||1x|
|2.5 mM dNTPs||4 μl||0.2 mM|
|LA Taq DNA polymerase||0.5μl||2.5 unit|
|ddH2O to final volume||50μl||Not applicable|
94°C 3 min
94°C 30 sec
55°C 30 sec 30 cycles
72°C 1 min
72°C 5 min.
|PC08||LA Taq DNA Polymerase||Long PCR Taq DNA Polymerase, 5U/μl|