Description

Thermostable T7 RNA Polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5′-3′ direction. And it can be transcribed efficiently in vitro at 50°C. In the process of in vitro transcription, it can improve the efficiency of capping and eliminate the production of dsRNA by-products In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99kDa.

Source

Recombinant E. coli

Concentration and Size

50U/μL or customized

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmoles of rCTP into acid-insoluble product in 1 hour at 37°C.

Application

1. Single stranded RNA synthesize;
2. High specific RNA probes synthesize;
3. The precursor of siRNA synthesize;
4. To make the precursor of RNA splicing reaction (RNA splicing);
5. Prepare the Capped mRNA

Storage Buffer

20mM Tris-HCl PH 7.9, 100mM NaCl, 10mM DTT, 0.1% Triton X-100, 1mM EDTA, 50%(v/v) Glycerol.

Buffer customized acceptable

Transportation

on blue ice

Storage

at -20°C for 24 months

Order

TR02

Thermal stable T7 RNA Polymerase

50U/μl