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Thermostable T7 RNA Polymerase

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Thermostable T7 RNA Polymerase is a monomeric bacteriophage encoded DNA directed RNA polymerase which catalyzes the formation of RNA in the 5'-3' direction. And it can be transcribed efficiently in vitro at 50°C. In the process of in vitro transcription, it can improve the efficiency of capping and eliminate the production of dsRNA by-products In the process of initiation of transcription T7 recognizes a specific promoter sequence, the T7 promoter. T7 consists of 883 amino acids and has a molecular weight of 99kDa.


Recombinant E. coli

Concentration and Size

50U/μL or customized

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmoles of rCTP into acid-insoluble product in 1 hour at 37°C.


  1. Single stranded RNA synthesize;
  2. High specific RNA probes synthesize;
  3. The precursor of siRNA synthesize;
  4. To make the precursor of RNA splicing reaction (RNA splicing);
  5. Prepare the Capped mRNA

Storage Buffer

20mM Tris-HCl PH 7.9, 100mM NaCl, 10mM DTT, 0.1% Triton X-100, 1mM EDTA, 50%(v/v) Glycerol.

Buffer customized acceptable


on blue ice


at -20°C for 24 months


TR02Thermal stable T7 RNA Polymerase50U/μl

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