N1-Me-Pseudo UTP 100mM Solution
2021-12-24
T7 RNA Polymerase
2021-12-24
Description
T7 high yield transcription kit optimized the transcription reaction system. T7 RNA polymerase synthesized RNA complementary to one strand of DNA from the downstream of template DNA T7 promoter, and simply and quickly obtained a large number of RNA molecules.
One reaction of this kit can be transcribed to produce 150-200μg RNA, transcribed and synthesized RNA can be used for downstream applications such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.
Storage
-20°C, or -80°C for a long time.
Components
| Component | Volume |
| Enzyme Mix | 50μl |
| 10×Transcription Buffer | 200μl |
| ATP (100 mM) | 80μl |
| UTP (100 mM) | 80μl |
| GTP (100 mM) | 80μl |
| CTP (100 mM) | 80μl |
| Control template（100ng/μl | 10μl |
| Lithium Chloride Precipitation Solution | 1.5ml |
| DNase I, RNase-free(1U/μl) | 50μl |
| RNase Free Water | 1ml |
Applications
- Synthesis of single stranded RNA;
- Synthesis of highly specific RNA probes;
- Synthetic siRNA precursor;
- The precursor of RNA splicing was prepared.
Order
| E131 | T7 High Yield RNA Transcription kit | Output 150-200μg RNA per reaction, 50rxn/kit. RNA can be used for downstream applications such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation |
