N1-Me-Pseudo UTP 100mM Solution
2021-12-24
T7 RNA Polymerase
2021-12-24
Product Number: E131
Shipping and Storage
-20℃
Component
| Component | Volume |
| Enzyme Mix | 50μl |
| 10×Transcription Buffer | 200μl |
| ATP (100 mM) | 80μl |
| UTP (100 mM) | 80μl |
| GTP (100 mM) | 80μl |
| CTP (100 mM) | 80μl |
| Control template（100ng/μl） | 10μl |
| Lithium Chloride Precipitation Solution | 0.75ml×2 |
| DNase I, RNase-free(1U/μl) | 50μl |
| RNase Free Water | 1ml |
Description
As biological macromolecules, mRNA can be synthesized on a large scale through in vitro transcription (IVT,in vitro transcription). The T7 promoter is currently one of the most efficient promoters for transcription, so using T7 RNA polymerase (TR01,T7 RNA Polymerase) for in vitro transcription can easily and quickly obtain a large number of RNA molecules.This kit has been optimized through a series of transcription reaction systems, and RNA complementary to a single strand in DNA is synthesized from downstream of the template DNA T7 promoter using T7 RNA Polymerase. The operation is simple and fast.
This reagent kit can transcribe and produce 150-200μg RNA in one reaction. The synthesized RNA can be used for downstream applications such as RNA structure and function research, RNA enzyme protection, probe hybridization, RNAi, microinjection, and in vitro translation.
Application
- Synthesis of single stranded RNA;
- Synthesis of highly specific RNA probes;
- Synthesis of siRNA precursors;
- Produce precursors for RNA splicing reactions.
Order
| E131 | T7 High Yield RNA Transcription kit | Output 150-200μg RNA per reaction, 50rxn/kit. RNA can be used for downstream applications such as RNA structure and function research, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation |
