HiFi Long PCR MasterMix
2026-01-20T7 RNA Polymerase 200 U/μl
Product Number: TR03
Shipping and Storage
Store at -20°C℃±5℃.
Component
| Component | TR03 |
| T7 RNA Polymerase | 5KU |
Description
As a biomacromolecule, mRNA can be synthesized at scale through in vitro transcription (IVT). The T7 promoter is currently one of the most efficient promoters for transcription, enabling rapid and straightforward production of large quantities of RNA molecules using T7 RNA polymerase. As a byproduct of IVT, double-stranded RNA (dsRNA) can be recognized by corresponding nucleic acid receptors, triggering innate immune inflammatory responses that severely impact the efficacy of mRNA vaccines. Strict control during production is essential. This product employs a T7 RNA polymerase engineered through molecular evolution, significantly reducing dsRNA levels in transcription products and lowering the immunogenicity of synthesized mRNA.
This product is a GMP-grade recombinant T7 RNA polymerase produced through large-scale fermentation using Escherichia coli. It is manufactured with pharmaceutical-grade raw materials and excipients, with strict control over host protein residues, nucleic acid residues, and other impurities. The production and quality management processes comply with GMP standards, ensuring traceability of the entire production process and all raw materials.
Application
- Synthesize single stranded RNA for the preparation of mRNA vaccines and other applications.
- Synthesize highly specific RNA probes.
- Synthesize siRNA precursor.
- Preparation of RNA splicing precursors.
- Synthesize capped RNA using cap analogues.
Quality control
| Project | Standard |
| Appearance | Clear liquid |
| Identification | Should be positive |
| Visible foreign matter | In compliance with regulations |
| pH | 7.3-7.7 |
| Activity | 180-220U/μL |
| Purity | ≥95% |
| Protein content | In compliance with regulations |
| Residual endonuclease | The degradation of substrates shall not exceed 10% |
| Residual exonuclease of nucleic acid | The degradation of substrates shall not exceed 10% |
| RNA enzyme residue | The degradation of substrates shall not exceed 10% |
| Bacterial endotoxin | < 5EU/mL |
| Exogenous DNA residue | ≤ 100pg/mg |
| Residual bacterial protein | ≤ 50ppm |
| Mycoplasma | Negative |
| Heavy metal | ≤ 10ppm |
| Microbial Limit | The total number of aerobic bacteria should not exceed 1cfu/10mL, and the total number of mold and yeast should not exceed 1cfu/10mL |
Storage buffer solution
50mM Trizma base; 100mM NaCl; 1mM EDTA; 20mM β-ME; 50% (v/v) Glycerol; 0.1% Triton X-100; pH 7.5.
Source
E.coli carrying bacteriophage T7 RNA polymerase gene
Features
It exhibits high specificity for the T7 promoter.
Definition of active units
The enzyme amount required to incorporate 1nmol of [3H] GMP into an acid insoluble precipitate within 1 hour at 37℃ and pH 8.0 is defined as 1 active unit.
Related Products
TR02 – Thermostable T7 RNA Polymerase
TR03 – T7 RNA Polymerase 200U/ul
GMP-T701 – T7 RNA Polymerase, GMP Grade
E131 – T7 High Yield RNA Transcription kit
