S-adenosylmethionine is the most important methyl donor in vivo and an auxiliary substrate involved in methyl transfer reaction. In the synthesis of mRNA in vitro,both the hydroxyl group of ribose and the amino group of base need the participation of SAM during methylation.
The SAM is prepared in 0.005M H2SO4 and 10% EtOH solution, filtered to provide in the form of aseptic liquid. In the process of mRNA capping, the combination of SAM and methyltransferase can transform the Cap0 structure of the 5′ cap region into Cap1 structure.
|Appearance||Clear colorless solution|
|DNase testing||Not detectable|
|RNase testing||Not detectable|
|Microbial residue||Not detectable|
|GMP-SAM01||S-adenosylmethionine (SAM)||GMP Grade, Purity>90%(75%S,S), 32mM solution. Size 1ml, 20ml, 100ml, 500ml|