CRISPR/Cas9 is an adaptive immune defense system formed by bacteria and archaea during their long-term evolution. The CRISPR/Cas9 system integrates fragments of invading phage and plasmid DNA into the CRISPR sequence and utilizes corresponding CRISPR RNAs (crRNAs) to guide the degradation of homologous sequences by Cas9 proteins, thereby providing immunity. The artificially modified Cas9/sgRNA system uses sgRNA (short guide RNA) to guide Cas9 proteins to recognize and cleave double stranded DNA with sgRNA targets, which can be used for gene knockout and precise DNA editing operations. The Cas9 nucleic acid Endonuclease provided by this product can enable the protein to enter the nucleus for Genome editing by adding a nuclear localization signal (NLS) to the N-terminal of the protein. This product is a pure protein system to avoid plasmid or virus interference during CRISPR gene knockout.
|Component||CAS09N-01 (500 pmol)||CAS09N-02 (1000 pmol)|
|Cas9 NLS (20 μM)||25 µl||50 µl|
|Cas9 NLS Reaction Buffer (10×)||1 ml||2 ml|
Store at -30 ~ -15 ℃. Transportation conditions: ≤ 0 ℃.
1 unit refers to the amount of enzyme required to add 0.5 pmol of dNTP to the acid insoluble precipitate during a 10 minute reaction at 30 ℃.
Obtained through E. coli recombination, expression, and purification, the expressed gene is derived from Streptococcus pyogenes.
Cell gene editing, in vitro screening of efficient gRNA sequences, specific double stranded DNA cleavage guided by gRNA, selective linearization of double stranded DNA containing specific sequences, etc.