LbaCas12a (Cpf1) Nuclease recognizes T-rich TTTN PAM sequences, opening up additional genomic regions for gene targeting. The required gRNA is short, with only 40-44 bases. It has two nuclear localization signals, improving the efficiency of transportation to the nucleus. The cutting product is a 5 ‘protruding end. The activity temperature range of this enzyme is between 16 ℃ and 48 ℃. Compared with the homologous enzyme of Acidaminococcus, it is still active at lower temperatures and can be used to edit the genome of cold-blooded animals, such as Zebrafish and African clawed frog. High concentration enzymes can be used for micro injection, electroporation, and liposome transfection.
|Component||CAS12A-01 (100 pmol)||CAS12A-02 (1000 pmol)|
|LbaCas12a (1 μM)||100 µl||1000 µl|
|LbaCas12a Reaction Buffer (10×)||1 ml||2 ml|
Store at -30 ~ -15℃. Transportation conditions: ≤ 0 ℃.
1 unit refers to the amount of Cas12a enzyme required to cleave 1 pmol ssDNA probe within 1 minute under 37 ℃ reaction conditions.
Obtained through E. coli recombination, expression, and purification, the expressed gene is derived from Lachnospiraceae bacterium.
In vitro screening of efficient gRNA sequences, specific double stranded DNA cleavage guided by gRNA, selective linearization of double stranded DNA containing specific sequences, etc.