Manual

Product Number: CAS12B

Shipping and Storage

Store at -30～-15 ℃ and transport at ≤ 0 ℃.

Components

Description

AacCas12b nuclease (C2c1) is an RNA mediated endonuclease that can specifically cleave the target double stranded DNA in the presence of PAM (TTN), causing the DNA double stranded to break and generate sticky ends.And AacCas12b specifically cleaves single stranded DNA targets without relying on PAM sequences. In addition, both double stranded and single stranded DNA targets can activate the trans cleavage activity (i.e., bypass cleavage activity/accessory cleavage activity) of AacCas12b. When AacCas12b enzyme binds to sgRNA and target DNA to form a ternary complex, it will activate the trans cleavage activity against non-specific sequence ssDNA, chopping any sequence of ssDNA in the system.

AacCas12b is derived from the acidophilic and heat-resistant bacterium Alicyclobacillus acidoterrestris, and the optimal shear reaction temperature is 48 ℃.AacCas12b relies on the guidance of crRNA and tracrRNA (or sgRNA formed after connection).In addition, AacCas12b can be applied not only to the cleavage of target dsDNA, but also to the rapid detection of target nucleic acids through its trans cleavage activity against ssDNA.

Source

Obtained through E.coli recombination, expression, and purification, the expressed gene is derived from Alicyclobacillus acidoterrestris.

Application

Used for the cleavage of target dsDNA, the trans cleavage activity of ssDNA can also be used for rapid detection of target nucleic acids.

Unit definition

1 unit refers to the amount of Cas12b enzyme required to cleave 1 pmol ssDNA probe within 1 minute under reaction conditions of 48℃.