Manual

Product Number: BS03

Shipping and Storage

Store at -20°C.

Components

Description

WellStart III BST DNA polymerase is a hybrid enzyme containing WarmStafi Bst DNA polymerase and a thermostable reverse transcriptase. WarmStafi Bst III features modifications over Bst 3.0, eliminating nonspecific amplification during room-temperature reaction setup and eliminating the need for a separate activation step, thereby enhancing reaction specificity. The thermostable reverse transcriptase is a genetically engineered novel enzyme with significantly improved cDNA synthesis speed and thermal stability, capable of tolerating reaction temperatures up to 60°C, making it suitable for reverse transcription reactions with RNA templates possessing complex secondary structures. WellStart III BST DNA polymerase can be applied to isothermal amplification reactions (LAMP/RT-LAMP) using RNA or DNA as templates.

Application

This product is suitable for various isothermal amplification reactions such as RT-LAMP, LAMP, RCA, CPA, etc.

Thermal inactivation

Incubate at 80 ℃ for 5 minutes to inactivate.