Manual

Product Number: BS07

Shipping and Storage

Transportation under 0°C and storage at -25~ -15°C.

Component

Description

Bst DNA polymerase V2 is derived from Bacillus stearothermophilus DNA Polymerase I, which has 5´→ 3´ DNA polymerase activity and strong chain replacement activity, but no 5´→3´ exonuclease activity. Bst DNA Polymerase V2 is ideally suitable for strand-displacement, isothermal amplification LAMP (Loop mediated isothermal amplification) and rapid sequencing. Well Start Bst DNA polymerase,large fragment is a hot-start version based on Bst DNA polymerase V2 obtained by reversible modification technology, which can inhibit DNA polymerase activity at room temperature, so the reaction system can be operated and formulated at room temperature to prevent non-specific amplification and improve reaction efficiency, and this version can be lyophilized. In addition, its activity is released at high temperatures, so there is no need for a separate activation step.

Application

1. LAMP isothermal amplification;
2. DNA strand single displacement reaction;
3. High GC gene sequencing;
4. DNA sequencing of nanogram level.

Unit Definition

One unit is defined as the amount of enzyme that incorporate 25nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Heat Inactivation

80℃, 20 min.