Manual

Product Number: BS05

Shipping and Storage

-20℃.

Components

*The reaction buffer contains 40mM Mg

Description

BST 2.0 DNA Polymerase is a homolog of Bacillus stearothermophilus DNA polymerase (BST DNA Polymerase, Large Fragment), derived from E.coli strain. Obtained through multiple purification and isolation after expression in Escherichia coli using gene recombination technology. This enzyme has 5′→ 3′ DNA polymerase activity, but lacks 5′→ 3′ exonuclease activity. Compared with wild-type BST DNA polymerase large fragments, BST 2.0 DNA polymerase exhibits higher amplification speed, yield, and sensitivity.

Features

1. Compared with large fragments of BST DNA polymerase, the amplification speed and sensitivity have been significantly improved.
2. Optimization was carried out for loop mediated isothermal DNA amplification (LAMP).
3. Strong chain displacement activity.

Application

1. DNA sequencing rich in GC sequences;
2. Rapid sequencing of trace (nanogram) DNA templates;
3. Random primer DNA labeling;
4. Double stranded DNA 5 ‘protruding end patch labeling method;
5. It can be used for isothermal DNA amplification, such as loop mediated isothermal amplification (LAMP), whole genome amplification (WGA), helicase isothermal gene amplification (HDA), etc.

Unit definition

The amount of enzyme required to add 25nmol of dNTPs to acid insoluble precipitate within 30 minutes at 65 ℃ is defined as one active unit.