HotStar PCR Mix High Specification, Ready to use HotStar PCR reagent no primer dimer

HotStar Taq Master mix with blue dye

Chemical modified DNA polymerase

Description

Include the HotStar DNA Polymerase, PCR Buffer, Mg²⁺, dNTPs, the blue dye and the stabilizing agent.
Has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues.
Inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.
Activated in 10 minute incubation at 95°C.
The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′-5′ exonuclease activity.
The extending speed is 1 kb/30 sec. There is an "A" on 3′ end. The PCR product can be cloned in TA vector.

Applications

Hot-start PCR amplification Specific amplification of complex cDNA and genomic template Amplification from low copy number DNA template Real-Time PCR Multiple PCR Generation of PCR products for TA cloning

Quality Control

• Functional absence of double and single-stranded endonuclease activity;
• Assayed for amplification from as little as 10 ng of human genomic DNA;
• Retain full activity at room temperature for one week;
• No host DNA residue.

Storage

Store at -20°C

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