2x E Taq PCR Master Mix Lyophilized Powder
2x SYBR qPCR Master Mix no rox
Show all

HotStar PCR Master Mix

Datasheet | MSDS | COA | Blog | Request

HotStar PCR Mix High Specification, Ready to use HotStar PCR reagent no primer dimer

HotStar Taq Master mix with blue dye

Chemical modified DNA polymerase


Include the HotStar DNA Polymerase, PCR Buffer, Mg2+, dNTPs, the blue dye and the stabilizing agent.
Has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues.
Inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.
Activated in 10 minute incubation at 95°C.
The activated enzyme maintains the same functionality as Taq DNA polymerase: Has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′-5′ exonuclease activity.
The extending speed is 1 kb/30 sec. There is an "A" on 3′ end. The PCR product can be cloned in TA vector.


Hot-start PCR amplification Specific amplification of complex cDNA and genomic template Amplification from low copy number DNA template Real-Time PCR Multiple PCR Generation of PCR products for TA cloning

Quality Control

  • Functional absence of double and single-stranded endonuclease activity;
  • Assayed for amplification from as little as 10 ng of human genomic DNA;
  • Retain full activity at room temperature for one week;
  • No host DNA residue.


Store at -20°C


PCM132×Hotstar PCR Master MixChemical modified Hotstar DNA Polymerase PCR Mix, without dye. 1ml/vial or bulk
PCM13B2×Hotstar PCR MasterMix, with blue dyeChemical modified Hotstar DNA Polymerase PCR Mix, with blue dye. 1ml/vial or bulk

Get a quote now