HotStar TaqMan Mixture (With ROX) is a convenient premixed 2x concentrated solution applied to probe-based real-time PCR. Which include the HotStar DNA Polymerase, PCR Buffer, MgCl2, dNTPs, ROX and the stabilizing agent. It can be applied to various probes and target sequence detection of genomic DNA and cDNA such as gene expression analysis, gene copy number analysis and SNP genotyping etc. The HotStar Taq DNA polymerase should be activated by incubation at 95°C for 10 minutes to effectively avoid the non-specific amplification caused by primer dimer or the non-specific combination of primer and template at room temperature. The combination of unique PCR buffer and hot start enzyme can effectively inhibit non-specific product and significantly improve the amplification efficiency, and can detect the single copy template. The kit is applied to a wide linear range and can exactly quantitate the target gene. The ROX is a dye molecule that can be used to normalize the well-to-well fluorescent fluctuations and can be applied to all the qPCR instruments using ROX as calibration dye.
1ml or Bulk
Gene expression analysis
Gene copy number analysis
High sensitivity: precisely quantitate low copy template
High specificity: maximatily avoid the production of primer dimers and nonspecific product
High amplification efficient; Lower CT value
Shipping And Storage
Store in dark at -20°C for up to 1 year.
For frequent use, Store at 2-8°C for short-term (1 week), and avoid freeze thawing.
|PCM39||2x TaqMan qPCR Master Mix(no ROX)||TaqMan probe qPCR Mix, Hotstar tech, without ROX|
|PCM40||2x TaqMan qPCR Master Mix(with ROX)||TaqMan probe qPCR Mix, Hotstar tech, with ROX|