Manual

Pfu DNA Polymerase, 5U/μL

Product Number: PC0301

Shipping and Storage

-20°C

Component

Description

This enzyme is a high fidelity heat-resistant DNA polymerase, with a fidelity 8-10 times that of Taq DNA polymerase. This enzyme not only has 5′-3′DNA polymerase activity, but also has 3′-5′exonuclease proofreading activity, which can timely remove nucleotide mismatches in the DNA synthesis chain, greatly increasing the accuracy of base pairing and ensuring the high fidelity of DNA synthesis. This enzyme does not have 5′-3′exonuclease activity.

Source

E. The recombinant strain of Escherichia coli contains genes cloned from Pyrococcus furiosus.

Definition of Activity

The amount of enzyme that can catalyze the polymerization of 10nmol dNTP into a nucleotide fragment by reacting calf thymus DNA as a template at 72℃ for 30 minutes is defined as one active unit (U).

Storage buffer

 [PH8.0] 20mM Tris HCl, 100mM KCl, 0.1mM EDTA, 0.1% NP-40 (v/v), 0.1% Tween-20 (v/v), 1mM DTT, 50% glycerol (v/v).

Reaction buffer

[10 × Pfu Buffer (containing Mg²⁺）] 200mM Tris-HCl（PH 8.8）, 100mM KCl, 160mM(NH4)2SO4，1 % Triton X-100，1 mg/ml BSA, 20mM MgSO4.