Manual

Product Number: TN5T02

Shipping and Storage

Ship on dry ice; store at −80℃. Valid for 6 months. Avoid repeated freeze‑thaw cycles. Store transposon DNA at −80℃, valid for 1 year.

Components

Description

Tn5 Transposase is a highly‑active engineered mutant Tn5 transposase derived from E. coli. It efficiently mediates random insertion of Tn5 transposons into target DNA sequences, exhibiting high transposition insertion efficiency against both eukaryotic and prokaryotic DNA. This product specifically recognizes DNA fragments flanked by Mosaic End (ME) sequences (including ME‑containing primers) to assemble Tn5 transposomes. The assembled transposomes bind randomly to target DNA, cleave the DNA, and insert their cargo DNA fragment. Tn5 transposase is widely applied in in‑vitro transgenesis (integration of exogenous genes into host cells) and Next‑Generation Sequencing (NGS) library construction.

Application

This product can be used for DNA fragmentation and adapter tagging during NGS library preparation; incorporation of sequencing primers into cloned DNA or plasmids; construction of bacterial gene‑knockout libraries; engineering of novel bacterial strains; insertional inactivation of target genes; insertion of T7 transcription promoters, antibiotic resistance markers and other elements into target DNA, etc.

Unit Definition

Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).

Specification

1. Source: Recombinant E. coli strain harbouring the Tn5 gene.
2. 5× Stop Buffer: 50mM EDTA (pH 8.0).