Tn5 Transposase
2026-08-20Fast T4 DNA Ligase
Product Number: LG02
Shipping and Storage
Ship with ice packs; store at −20℃. Valid for 2 years. Avoid repeated freeze‑thaw cycles.
Components
| Component | LG02 12KU |
| Fast T4 DNA Ligase (600 U/μl) | 20μL |
| 2×Fast Ligase Buffer | 1mL |
| 10×T4 DNA Ligase Buffer | 200μL |
Description
T4 DNA Ligase catalyses the formation of phosphodiester bonds between adjacent 5’‑phosphate termini and 3’‑hydroxyl termini on double‑stranded DNA or RNA. This enzyme can catalyse ligation between blunt‑end or sticky‑end fragments; it can also repair nicks within double‑stranded DNA, RNA, or DNA/RNA hybrid duplexes.
Application
Ligation of DNA inserts with vector DNA; ligation of DNA fragments with linkers or adapter DNA.
Unit definition
One unit (U) is defined as the amount of enzyme required to ligate greater than 50 % of DNA fragments from λDNA‑Hind III digest in a 20μL ligation system containing 6 μg substrate, incubated at 16℃ for 30 min.
