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2x SYBR qPCR Master Mix separate rox

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2x Ultra SYBR Mixture (With separate ROX) is a convenient premixed 2x concentrated solution for SYBR Green I-based qPCR which include HotStar Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dyes, Mg2+. It can be applied to the target sequence detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes. The modified HotStar Taq DNA polymerase is inactive under 40°C which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of prime and template. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. The ROX is a dye molecule that can be used to normalize the well-to well fluorescent fluctuations and can be applied to all qPCR instruments using ROX as calibration dye. It has a higher sensitivity and a wider linear range which can exactly quantitate the target gene or low copy template.


2x SYBR qPCR mix 1.25ml or bulk
Rox reference dye (50µmol) 25ul


Gene expression analysis
Gene copy number analysis


High sensitivity: exactly quantitate the low copy template
High specificity: maximatily reduce the primer dimers and non-specific product
Wide linear range; Accurate quantitation

Shipping And Storage

Store in dark at -20°C for up to 1 year.
For frequent use, Store at 2-8°C for short-term (1 week), and avoid freeze thawing.

Rox reference dye user guide

InstrumentROXFinal Concentration
Per 50µl PCR Reaction
ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus0.5 µl(0.3-0.5µl)500 nmol(300-500nmol)
ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett Rotor Gene 30000.05 µl*  (0.03-0.05µl)50 nmol(30-50nmol)


PCM33B2x Ultra Sybr qPCR Mix, with separate ROXSYRB qPCR Mix, Hotstar tech, with separate ROX(100x)

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