Not I, NotI
SP6 RNA Polymerase
Show all

Duplex specific nuclease

Datasheet | MSDS | COA | Blog | Request


Duplex specific nuclease (DSN) is a specific nuclease enzyme, which has strong activity to hydrolyze DNA in dsDNA or DNA-RNA hybrid duplexes. The DSN has very slight activity for hydrolysis ssDNA or RNA.


Recombinant E. coli


1U/μL or customized


Homogenization of Full-length cDNA Library

Single nucleotide polymorphism (SNP) detection

high-throughput sequencing

Detection of microRNAs (miRNAs)


purity>95% by SDS PAGE


Store at -20°C

Unit Definition

Under the standard reaction system, the increase of 0.001 per minute in the absorbance of 50 𝜇g/ml calf thymus DNA at 25 ℃ is defined as 1 unit.


Duplex specific nuclease50U100U1000U
10xDSN Reaction Buffer0.5ml0.5ml3ml
2×DSN Stop Buffer1ml1ml6ml


1. Prepare the reaction according following table on ice.

50-500 ng DNAx μl
10×DSN Reaction Buffer1 μ
DSNy μl
ddH2O up to10 μl

2. Mix gently and centrifugate briefly.

3. Incubate at 65 ℃ for 7-20 min.

4. Add 5 μl 2 × DSN Stop Buffer, gently mixed, centrifuged briefly, 65 ℃, incubated for 5 min to terminate the reaction.


1. DSN digestion time depends on the sample and specific experimental requirements. The incubation temperature can vary from 35 to 70 ℃; In this case, the incubation time and DSN concentration need to be optimized separately.

2. DSN is active in the presence of divalent cations (Mn2+, Co2+ or Mg2+). The concentration of Mg2+ions in most applications should be at least 5 mM. EDTA can inhibit DSN activity.

3. The optimum temperature for DSN activity is 60 ℃. However, DSN retains only 10% activity at 80 ℃.

4. DSN is resistant to protease K treatment (incubation at 37 ℃ for 30 minutes).

5. DSN is highly sensitive to salt ion concentration (for example, the activity decreases by 10 times under 0.2M NaCl.


DSN01Duplex specific nuclease1U/ul Specific nuclease enzyme hydrolyze DNA in dsDNA or DNA-RNA

Get a quote now