Manual

Product Number: PCK85M

Storage

Store at -20 ℃ and transport on dry ice.

Components

Description

This kit is suitable for both Illumina and MGI sequencing platforms. It provides the premixed enzyme modules required for DNA end repair,5′-end phosphorylation modification,3′-end A addition and Adaptor ligation in DNA library preparation. It can be used with different sequencing platform adapters. Primer kits can prepare DNA into specific DNA libraries for Illumina or MGI sequencing platforms. Using high-fidelity DNA polymerase for library enrichment and unbiased PCR amplification,the coverage area of the sequence is expanded,and high-quality DNA libraries can be prepared. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability of library preparation to the greatest extent.

Features

1. End repair and phosphorylation and adding A to complete in one step;
2. After the end repair without purification,directly add the Adaptor;
3. Ultra fidelity amplification,the maximum extent to reduce the amplification bias;
4. The library is suitable for multiple sequencing platforms:MGI sequencing platform:MGISEQ-2000, MGISEQ-200, BGISEQ-500 and other MGI sequencing platform lllumina GAllx, HiSacnSQ, HiSeq 250/2000/1000, MiSeqsequencing and other lllumina platform sequencers;
5. It is suitable for preparing gDNA and cDNA libraries after physical interruption.

Schematic diagram of DNA library construction process