Manual

Description

S-adenosylmethionine is the most important methyl donor in vivo and an auxiliary substrate involved in methyl transfer reaction. In the synthesis of mRNA in vitro,both the hydroxyl group of ribose and the amino group of base need the participation of SAM during methylation.

Feature

The SAM is prepared in 0.005M H₂SO₄ and 10% EtOH solution, filtered to provide in the form of aseptic liquid. In the process of mRNA capping, the combination of SAM and methyltransferase can transform the Cap0 structure of the 5′ cap region into Cap1 structure.

Specification

Customized Spesfication:

1）Endonuclease Activity (Nicking) – A 50 µl reaction in Buffer 2 containing 1 µg of supercoiled PhiX174 DNA and a minimum of 5 µl of S-adenosylmethionine (SAM) incubated for 4 hours at 37ºC results in <20%conversion to the nicked form as determined by agarose gel electrophoresis.

2）Non-Specific DNase Activity (16 Hour) – A 50 µl reaction in NEBuffer 2 containing 1 µg of PhiX174-HaeIII DNA and a minimum of 5 µl of S-adenosylmethionine (SAM) incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.

3）Restriction Digest (CpG Resistant, SAM) – A 20 µl reaction in 1X Buffer 2 containing 1 µg of LambdaDNA, 1 unit of M. SssI (CpG Methyltransferase), and 160 µM S-adenosylmethionine (SAM) is incubated for 1hour at 37°C. The resulting DNA is resistant to digestion with BstUI as determined by agarose gel electrophoresis

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