Manual

Product Number: RESA04

Shipping and Storage

Stored at -20℃, valid for two years.

Component

Description

The rapid endonucleases produced by our company are a series of high-quality restriction endonucleases that have undergone genetic engineering recombination and can quickly complete DNA enzyme cleavage within 5-15 minutes using only one buffer solution.

Application

SapI rapid endonuclease is suitable for rapid enzymatic digestion of plasmid DNA, PCR products, or genomic DNA.

Features

1. Enzymatic cleavage can be completed within 5-15 minutes;
2. All endonucleases share one enzyme digestion buffer, CutEZ™ Buffer, Greatly simplifies the enzyme digestion reaction system, making it convenient for double enzyme digestion or multi enzyme digestion;
3. Targeting different enzymes in CutEZ™ The problem of differences in activity in Buffer has been addressed by adjusting the concentrations of different enzymes. Therefore, the enzyme digestion reaction can be carried out uniformly at a dosage of 1μL enzyme per 20μL system;
4. Alkaline Phosphatase, Antarctic Phosphatase, T4 DNA Ligase, T4 Polynucleotide Kinase, T4 PNK (3′ phosphatase minus) Many modified enzymes are 100% compatible with CutEZ ™ Buffer, Make reaction systems such as’ enzyme cleavage linking ‘and’ enzyme cleavage modification linking ‘compatible and support one tube reactions;
5. Good enzyme activity redundancy makes it easy to cope with substrate excess or difficult template enzyme digestion.