Manual

Product Number: CK04504

Characteristics:

Source: Chinese Hamster Ovary cell line
Description: Human Transferrin (Val20-Pro698)
Accession # P02787
Predicted molecular mass: 75.2 kDa

Specification:
Appearance: White powder, Colorless to light red solution with no opalescence after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is ≤5 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Formulation: Lyophilized from a 0.2 μm filtered solution in sterile water.
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:
Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at 2°C to 8°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.