Manual

Product Number: TN5T01

Shipping and Storage

Store at -80℃. Transportation conditions: ≤ 0℃.

Components

Enzyme storage solution: 50mM HEPES (pH7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol.

Reaction Buffer (5×): 50mM HEPES (pH7.2), 500mM NaCl, 50mM MgCl₂.

Stop Buffer (5×): 50mM EDTA (pH8.0).

Description

PA-Tn5 Transposase， It is a novel fusion enzyme (pA-Tn5 Transposase) that combines Protein A with a modified ultra-high activity Tn5 transposase to form a dual activity enzyme. It can efficiently insert Tn5 transposons randomly into target sequences. PA-Tn5 Transposase can specifically recognize DNA fragments containing chimeric end sequences (ME) at both ends (including primers containing ME sequences), ultimately forming Tn5 Transposomes, which can randomly bind to target DNA and cleave and insert DNA fragments carried by it. Tn5 transposase is widely used in fields such as in vitro transgenic (integration of exogenous genes into host cells) and next-generation sequencing (NGS) library construction.

Application

This product can be used for fragmentation and adapter addition during the construction of next-generation sequencing (NGS) libraries; Introducing sequencing primers into cloned DNA or plasmids; Establishment of bacterial gene knockout library; Engineering transformation of new bacterial strains; Insertion inactivation of target genes; Insert T7 transcription promoter, resistance markers, etc. into target DNA, etc.

Unit Definition

PA-Tn5 transposase refers to the amount of enzyme required to completely cleave 1ug of DNA fragments containing recognition sequences under 37℃ conditions for 1 hour, defined as 1 unit (U).