Manual

Product Number: RT10

Shipping and Storage

Storage at -25~-15°C and transportation≤ 0℃. Avoid repeated freezing and thawing, and valid for 18 months.

Component

Description

X Reverse Transcriptase is  an  aptamer-modified  and RNA-template-dependent DNA polymerase that inhibits its reverse transcription activity below 40°C. X Reverse Transcriptase lacks 3′ → 5′ exonuclease activity and has RNase H activity. The enzyme can use RNA as a template to synthesize a complementary DNA strand, which can be applied to first-strand cDNA synthesis. Because of its high activity between 50-65°C, it is particularly suitable for RT-LAMP (Loop-Mediated Isothermal Amplification). Since the polymerase activity of X Reverse Transcriptase is inhibited below 40°C, the non-specific amplification caused by mismatching of primers during the preparation of the reaction system can be greatly reduced, solving the problems of primer-dimer formation before the reaction, improving the consistency and specificity of amplification.  X Reverse Transcriptase  supports high-throughput applications and operation at room temperature. X Reverse Transcriptase (Glycerol free) can be used to prepare lyophilized  preparations,  lyophilizable RT-LAMP reagents, etc.

Application

1. cDNA synthesis;
2. Combined with Bst DNA;
3. RT-LAMP isothermal amplification can be performed to detect target RNA.

Unit definition

One unit incorporates 1nmol of dTTP into acid-precipitable material in 20 minutes at 50°C using poly(A)•oligo(dT) as template-primer.