Manual

Product Number: PCK52

Storage condition

Store at -20°C .

Component

Description

The enhanced microRNA reverse transcription/fluorescence quantitative detection kit contains all the reagents for microRNA detection. This product adopts the Poly (A) tail addition method, using microRNA as a template, and using a specially optimized pre mixed microRNA RT Enzyme mix (including Poly (A) tail addition enzyme and reverse transcription enzyme) to efficiently complete cDNA synthesis through a one-step method of Poly (A) tail addition and reverse transcription; MicroRNA detection was performed using a 2 x microRNA qPCR mix. Suitable for samples containing microRNAs such as Total RNA or Small RNA.

Features

1. The optimal ratio of Poly (A) tail enzyme and reverse transcriptase, as well as the optimized reaction buffer, ensure the reverse transcription efficiency of microRNA.
2. PolyA tail addition and reverse transcription cDNA synthesis are completed in one step in the same tube.
3. 2×microRNA qPCR Mix has high amplification efficiency, strong specificity, and sensitivity.
4. Equipped with ROX Reference Dye, it can be used for various models that require high and low ROX reference dyes.