microRNA 1st Strand cDNA synthesis kit
2025-05-09microRNA RT & qPCR Detection kit
2025-05-12
Product Number: PCK51
Storage condition
Store at -20°C in the dark for at least 12 months, thoroughly dissolve and mix before use. 2×microRNA qPCR Mix (with Sybr Green) can be stored at 4°C for short-term use to avoid repeated freeze-thaw cycles. Reverse primer (10μM) should be stored at -20°C after each use.
Component
| Component | 125T×20μL |
| 2 ×microRNA qPCR Mix(With Sybr Green) | 1.25μL |
| Reverse primer(10μM ) | 55μL |
Description
The enhanced microRNA fluorescence quantitative PCR detection kit adopts SYBR The principle of Green I chimeric fluorescence method for microRNA fluorescence quantitative detection. This kit contains all the reagents for microRNA fluorescence quantitative detection, including 2×microRNA qPCR Mix and Reverse Primer. 2×microRNA qPCR Mix (including Sybr Green) is a new generation of pre mixed fluorescent quantitative PCR detection reagent specially developed for microRNA quantitative detection. The DNA polymerase used in it is an antibody modified hot start form, combined with a special buffer system, making the reaction more specific, sensitive, and able to accurately quantify over a wider range.
Note: This kit must be used in conjunction with the Enhanced microRNA Real-Time PCR Assay kit(PCK50).