hEPO mRNA (N1-Me-Pseudo UTP)
2025-11-14Super FiV M-MLV Reverse Transcriptase (RNase H-)
Product Number: RT07
Shipping and Storage
Store at -30 ~ -15 ℃ and transport on dry ice.
Components
| Component | RT07 10KU | RT07 200KU | RT07 2000KU |
| Super FiV M-MLV Reverse Transcriptase (RNase H-)（200U /μL) | 50 μL | 1 mL | 10 mL |
| 5×SuperRT Buffer | 1 mL | 10 mL | 100 mL |
Description
Super FiV M-MLV Reverse Transcriptase (RNase H-) is a reverse transcriptase that recombines and expresses mutated M-MLV genes in E. coli engineering bacteria. This enzyme can catalyze complementary DNA polymerization reactions using RNA or DNA: RNA hybrid chains as templates. The mutated Super FiV M-MLV Reverse Transcriptase (RNase H-) RNase H activity is missing, reducing RNA degradation in reverse transcription reactions and making it easier to obtain full-length cDNA. Super FiV M-MLV Reverse Transcriptase (RNase H-) exhibits excellent reverse transcription activity at 55 ℃ (the enzyme can be used for reverse transcription reaction at up to 60 ℃). For complex RNA structures, increasing the reverse transcription reaction temperature can significantly improve the yield of cDNA. In addition, the mutated Super FiV M-MLV Reverse Transcriptase (RNase H-) has better stability and can synthesize up to 15kb of cDNA. Suitable for the synthesis of first stranded cDNA, RT PCR, RT qPCR, and construction of full-length cDNA libraries.
Unit definition
Using Poly (A) as a template and oligo (dT) as a primer, the enzyme required to catalyze the addition of 1 nmol of dTTP within 10 minutes at 37 ℃ is defined as one active unit (U).
Quality control
200 U of this enzyme reacted with 1µg of 16S, 23S rRNA at 37 ℃ for 1 hour, and the electrophoresis band of the RNA remained unchanged.
