Manual

Product Number: RPA002

Shipping and Storage

Store at -20℃ to avoid repeated freezing and thawing.

Description

UvsY is a bacteriophage T4 recombinant regulatory protein that plays a promoting role in the homologous recombination process of UvsX. When searching for homologous sequences in UvsX, it needs to compete for binding sites with single stranded DNA binding proteins pre bound to single stranded DNA, and UvsY protein promotes this competition, which helps UvsX bind to single stranded DNA. It can enhance the DNA dependent ATPase activity of UvsX protein, reduce the minimum concentration required for its activity, and promote chain replacement. UvsY promotes the invasion of UvsX recombinase into ssDNA single stranded binding protein complex, leading to the release of single stranded binding protein, thereby promoting the binding of UvsX to single stranded DNA. This enzyme has no nuclease activity.

Our company provides gene recombinant expression of UvsY protein, with a molecular weight of~16KDa, high activity, and good stability. We constructed an efficient RPA isothermal amplification system using self-produced UvsY protein (as shown in the figure below).

A RPA isothermal amplification system was constructed using self-produced recombinant proteins such as Uxsx, UxsX, gp32, Bsu, etc.

M is a marker, and 1, 2, 3, and 4 are different RPA constant temperature amplification products.

Application

DNA isothermal amplification; RPA amplification; Genetic testing.

Specification

1. Dosage form: liquid or freeze-dried powder
2. Storage buffer: 50mM Tris HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 20% glycerol, pH 8.0
3. Molecular weight: Approximately 16 KDa (detected by SDS-PAGE)
4. Purity: ≥ 90% (detected by SDS-PAGE)
5. Thermal deactivation: 60℃, 10 minutes

Quality control

1. Detection of residual nucleases: Incubate 500U of this enzyme with 0.6μg of λ – Hind III at 37℃ for 16 hours, and the electrophoresis band of DNA remains unchanged.
2. Detection of residual endonuclease: 500U of this enzyme and 0.6μg Supercoiled pBR322 DNA were incubated at 37℃ for 4 hours, and the electrophoresis band of DNA remained unchanged.
3. E. coli DNA residue detection: The residual nucleic acid in 50U of this product was detected by TaqMan qPCR specific for E. coli 16s rDNA, and the E. coli genome residue was less than 10 copies.

Note

This reagent is only used for research and development or production, and is strictly prohibited from being used in human or animal experiments.