|Product Name||Proteinase K|
|Molecular Weight||28,900 D|
|Source||Mutated gene from Tritirachium album limber, Expressed in recombinant yeast.|
|Form||Lyophilized White Powder|
|Specific Activity||≥30U/mg dry weights|
Store at 4 to -20ºC recommended.
Mutated Proteinase K powder is stable at room temperature in 3 years.
Expiration: Three years if kept below 4ºC.
Dilution Buffer 1 (short time storage): 20 mM Tris-Cl (pH 7.4), 1 mM CaCl2, 15% glycerol, or d3H2O.
Dilution Buffer 2 (long term storage): 20 mM Tris-Cl (pH 7.4), 1 mM CaCl2, 50% glycerol, 0.02-0.1% Sodium azide.
The application of this Mutated Proteinase K is similar with wild type Proteinase K. But the mutated one has higher specific activity and more stable at RT. It is a broad substrate non-specific serine Proteinase. It is active over the pH range 4-11. It was used on any situation to digest native and denatured proteins. For instance, it is used for isolating mRNA or genomic DNA from different tissues and modifying glycoprotein for structure studies. Mutated Proteinase K is active with SDS, urea and EDTA and active between the temperature15-75ºC
|PK01||Proteinase K Lyophilized||Proteinase K Lyophilized Powder, Activity>=30U/mg protein|