Manual

Product Number: DDP0005-24T

Shipping and Storage

Store the kit at -20±5℃ with a shelf life of 12 months.

After thawing, the general master mix can be stored at 2~8°C for 1 week. Do not freeze and thaw repeatedly more than 3 times.

Components

Note: Components from different batch numbers cannot be mixed.

Description

Droplet digital PCR (ddPCR) enables absolute quantification without relying on standard curves or reference samples, and directly detects the copy number of target sequences. The principle is to partition a standard PCR reaction into numerous tiny reactors. Each reactor contains zero or multiple copies of target RNA templates, realizing single-molecule template reverse transcription and PCR amplification. After amplification, a biochip analyzer detects the fluorescence intensity of each droplet individually. Compared with negative droplets, positive droplets containing nucleic acids show enhanced fluorescence signals. Finally, the absolute copy number of target nucleic acids is calculated based on the proportion of positive droplets in accordance with the Poisson distribution.

This master mix contains DNA polymerase, dNTPs, magnesium chloride and buffer. Users only need to add appropriate primers, probes, reverse transcriptase, templates and nuclease-free water to perform one-step reverse transcription droplet digital PCR. The operation is simple and convenient.

Application

Mix this master mix with self-prepared primers and probes, and use it together with the droplet digital PCR system for pre-treatment prior to droplet generation.