Manual

Product Number: PH29P

Shipping and Storage

-20℃.

Components

Description

Phi29 Plus DNA polymerase is a protein engineered Phi29 DNA polymerase that is expressed in Escherichia coli and purified and isolated multiple times. Compared with wild-type Phi29 DNA polymerase, it has higher amplification efficiency and sensitivity, and can greatly shorten reaction time. Phi29 plus DNA polymerase has special chain displacement activity and efficient continuous synthesis properties, with strong binding ability to templates. It can continuously synthesize up to 70kb of DNA fragments without dissociating from the template. At the same time, the enzyme has a strong 3´→5´exonuclease correction function, with a fidelity 100 times higher than Taq DNA polymerase.

Application

1. Thermostatic protein prime DNA amplification;
2. Using random primers to amplify DNA;
3. Roll ring replication;
4. Copy extended region.

Features

1. Extremely high infiltration rate;
2. Super strong chain replacement capability;
3. High fidelity;
4. Reaction at moderate temperature;
5. Extremely high sensitivity and amplification efficiency.