The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase which has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme need to be activated in incubation at 95°C for 10 minute. The activated enzyme maintains the same functionality.
Taq DNA polymerase: Has the 5′ to 3′ DNA polymerase activity and 5′ to 3′ exonuclease activity without 3′ – 5′ exonuclease activity. The extending speed is 1 kb/30 sec. There is an “A” on 3′ end. The PCR product can be cloned in TA vector.
Concentration: 5 u/μl
Storage: Store at -20°C
|PC11||Hotstar DNA Polymerase||Anti body modifed Hotstar DNA Polymerase, suitable for IVD use|
|PC12||E star Taq DNA Polymerase||Double Anti body modifed Hotstar DNA Polymerase, suitable for IVD use|