Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.
M-MLV (200U/μl) 5×Buffer (with DTT)
Incubate at 37-52°C.
The yield and size of cDNA transcript increases with increasing amounts of RT.
Once thawed, store at -20°C. Repeated freeze thaw cycles will inactivate the enzyme. Aliquots can be stored for longer periods at -70°C.
Insect cells (Sf21) infected with baculovirus containing the pol gene of AMV.
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