Description:
Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.
Component:
M-MLV (200U/μl) 5×Buffer (with DTT)
Concentration:
200U/μl
Application:
Reaction Conditions:
Incubate at 37-52°C.
The yield and size of cDNA transcript increases with increasing amounts of RT.
Storage:
Once thawed, store at -20°C. Repeated freeze thaw cycles will inactivate the enzyme. Aliquots can be stored for longer periods at -70°C.
Source:
Insect cells (Sf21) infected with baculovirus containing the pol gene of AMV.
Delivery:
Faster delivery: Sample order in stock, and 3-7 working days for bulk orders after payment, shipping with blue ice