Description:

Avian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template.

Component:

M-MLV (200U/μl) 5×Buffer (with DTT)

Concentration:

200U/μl

Application:

• The first-strand cDNA synthesis
• RT-PCR
• cDNA Synthesis
• RNA Sequencing

Reaction Conditions:

Incubate at 37-52°C.

The yield and size of cDNA transcript increases with increasing amounts of RT.

Storage:

Once thawed, store at -20°C. Repeated freeze thaw cycles will inactivate the enzyme. Aliquots can be stored for longer periods at -70°C.

Source:

Insect cells (Sf21) infected with baculovirus containing the pol gene of AMV.

Delivery:

Faster delivery: Sample order in stock, and 3-7 working days for bulk orders after payment, shipping with blue ice