Manual

Product Number: RA07

Shipping and Storage

Can be stored for 2 years at -20℃ to avoid repeated freezing and thawing.

Component

Description

RNase III can cleave longer double-stranded RNA (dsRNA) into a group of small interfering RNAs (siRNAs) of varying sizes, ranging from 18-25bp, making it more suitable for RNA interference experiments in mammalian cells. Using 1.5 units (1μL) of ShortCut RNase III can completely digest 1μg of dsRNA into siRNAs.

The E. coli RNase III produced by our company is a recombinant protein expressed through recombinant methods and purified through multiple steps.

Feature

1. Provide siRNA for RNA interference research
2. Target determination
3. Remove dsRNAs
4. Preparation of effective siRNA suitable for any gene target
5. Heterologous siRNA can ensure silencing of target genes
6. From DNA template to transfection only takes one day
7. Avoiding the problem of repeatedly exploring experimental conditions when using synthetic siRNA.

Unit definition

1 active unit refers to the amount of enzyme required to cleave 1μg dsRNA into siRNA in a 1 × E. coli RNase III reaction buffer system at 37 ℃ for 20 minutes.

Activity determination conditions

1 ×RNase III reaction buffer: 50mM Tris HCl, 50mM NaCl, 1mM DTT (pH 7.5 @ 25℃). Add 20mM MnCl2 (provided with the product) and incubate at 37℃.

Quality control

After strict quality control testing, the product is ensured to have the highest activity and purity.

Enzyme storage buffer

500mM NaCl，10mM Tris-HCl，0.5mM EDTA，1mM DTT，50% Glycerol，pH 8 @ 25°C