Manual

Product Number: RE0585

Shipping and Storage

-20℃.

Components

Description

SspDI belongs to the conventional restriction enzyme series and can recognize G ^ GCGCC sequences. Unlike the Thunder series of rapid endonucleases, SspDI requires a longer time for enzyme cleavage to achieve complete cleavage of the DNA substrate, but this enzyme still uses the Thunder restriction enzyme universal reaction buffer FlashCut™ Buffer ， Can achieve dual enzyme digestion.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.