
FlashCut™ XhoI
2025-05-27
BsiWI
2025-05-27Product Number: RE0579
Shipping and Storage
-20℃.
Components
Component | Specifications |
SgeI (5U/μL) | 50μL |
10×SgeI Buffer | 1mL |
Description
SgeI can cleave DNA targets containing 5-methylcytosine on single or double stranded DNA. SgeI restriction endonuclease can recognize the m5CNNG (9/13) ^ site and has the best cleavage effect at 37°C in its unique buffer. To ensure consistent performance, the enzyme storage buffer contains pre mixed BSA, which enhances enzyme stability and binds to potential contaminants in DNA preparations.
Suggested reaction conditions
- 1×SgeI buffer solution;
- Incubate at 37℃;
- Prepare the reaction system according to the “DNA enzyme digestion process”.
Inactivation conditions
Incubate at 80℃ for 20 minutes.