Manual

Product Number: RE0579

Shipping and Storage

-20℃.

Components

Description

SgeI can cleave DNA targets containing 5-methylcytosine on single or double stranded DNA. SgeI restriction endonuclease can recognize the m5CNNG (9/13) ^ site and has the best cleavage effect at 37°C in its unique buffer. To ensure consistent performance, the enzyme storage buffer contains pre mixed BSA, which enhances enzyme stability and binds to potential contaminants in DNA preparations.

Suggested reaction conditions

1. 1×Sgel buffer solution;
2. Incubate at 37℃;
3. Prepare the reaction system according to the "DNA enzyme digestion process".

Inactivation conditions

Incubate at 80℃ for 20 minutes.