Manual

Product Number: RE0569

Shipping and Storage

-20℃.

Components

Description

FlashCut™  Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases in the universal FlashCut™  Or FlashCut™  Color Buffer has excellent activity and can complete enzyme digestion within 5-15 minutes. In addition, our company dephosphorylation and ligation reagents are available on FlashCut™  Buffer has 100% activity and supports one tube reaction, enhancing the experience of “enzyme digestion modification connection”.

FlashCut™  Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis.  FlashCut™  The migration rate of red dye of Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is close to each other; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × FlashCut™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the “DNA rapid enzyme digestion process”.

Inactivation conditions

Incubate at 80℃ for 20 minutes.