Manual

Product Number: RE0545

Shipping and Storage

-20℃.

Components

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both CutOne™ and CutOne™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on CutOne™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of "enzyme digestion modification connection".

CutOne™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of CutOne™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

1. 1 × CutOne™ Buffer solution.
2. Incubate at 37℃.
3. Prepare the reaction system according to the "DNA rapid enzyme digestion process".

Inactivation conditions

Incubate at 80℃ for 20 minutes.