tinzyme-endonuclease-exonuclease
FlashCut™ FspI
2025-05-26
tinzyme-endonuclease-exonuclease
FlashCut™ HhaI
2025-05-26
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FlashCut™ HaeIII

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tinzyme-endonuclease-exonuclease

Manual

Product Number: RE0533

Shipping and Storage

-20℃.

Components

ComponentSpecifications
FlashCut™ HpaII300μL
10× FlashCut™ Buffer2×1mL
10× FlashCut™ Color Buffer2×1mL

Description

FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of "enzyme digestion modification connection".

FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Suggested reaction conditions

  1. 1 × FlashCut™ Buffer solution.
  2. Incubate at 37℃.
  3. Prepare the reaction system according to the "DNA rapid enzyme digestion process".

Inactivation conditions

Incubate at 80℃ for 20 minutes.


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