Product Number: RE0526
Shipping and Storage
-20℃.
Components
Component | Specifications |
FlashCut™ EagI | 25μL |
10× FlashCut™ Buffer | 1mL |
10× FlashCut™ Color Buffer | 1mL |
Description
FlashCut™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All FlashCut™ Rapid endonucleases have excellent activity in both FlashCut™ and FlashCut™ Color Buffer, and can complete enzyme cleavage within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on FlashCut™Buffer has 100% activity and supports one tube reaction, enhancing the experience of "enzyme digestion modification connection".
FlashCut™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. The migration rate of red dye of FlashCut™ Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is similar; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.
Suggested reaction conditions
Inactivation conditions
Incubate at 80℃ for 20 minutes.