Manual

Product Number: CK03401

Characteristics:

Source: Pichia pastoris
Description: Human PDGF-BB (Ser82-Thr190)
Accession #: P01127
Predicted molecular mass: 12.2 kDa (monomer)

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin: ≤10 EU/mg by the LAL method
Activity: Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤5 ng/mL.

Formulation: Lyophilized from a 0.22 μm-filtered solution containing 100 mM Citrate-sodium citrate, 5% Mannitol, 0.1% Trehalose, pH 6.5

Size: 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability:

• 36 months at -20°C to -80°C in lyophilized state
• 6 months at -20°C to -80°C under sterile conditions after reconstitution
• 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.