Manual

Product Number: CK0701

Characteristics:

Source: E. coli
Description: Aprotinin (Arg36-Ala93)
Accession # P00974
Predicted molecular mass: 6.5 kDa

Specification:

Appearance: White powder, Colorless clear liquid after reconstitution
Purity: ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin: ≤100 EU/mg by the LAL method
Activity: ≥8000 KIU/mg. Trypsin unit: 1 μmol of N-benzoyl-L-arginine-ethylester (BAEE) that can be hydrolyzed per second is 1 trypsin unit.
Enzyme activity unit: The activity that can inhibit 1 trypsin unit is called 1 aprotinin activity unit (EPU).
Note: Another commonly used active unit is KIU (Kallikrein Inhibitor Unit). 1 EPU ≈ 1800 KIU.

Formulation: Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size: 50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution: It is recommended to redissolve in sterile deionized water.
Shipping: The product is shipped with blue ice.
Storage & Stability: 36 months at 2°C to 8°C in lyophilized state; 6 months at -20°C to -80°C under sterile conditions after reconstitution; 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.