Human NK Cell Expansion Super Kit
2025-12-10
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Human NK Cell Expansion Kit

Manual

Product Number: CK001

Shipping and Storage

Store at -20°C or below to avoid repeated freeze-thaw cycles. The validity period is 6 months.

Description

This document describes the recommended usage instructions for the Human NK Cell Expansion Kit for NK cell expansion. The kit contains three lyophilized products: K001-1A, K001-2A, and K001-3A. This kit is designed to be used with an initial culture medium. The specific usage recommendations are as follows:

Product Reconstitution

CK001-1A: Dissolve the lyophilized powder in 100μL of water, then add 7.4mL of PBS to prepare a coating solution; total volume is 7.5mL. Store at -20°C or below.
CK001-2A: Dissolve the lyophilized powder in 100μL of water, then add 9.9mL of initial culture medium to prepare an activation factor; total volume is 10mL. Store at -20°C or below.
CK001-3A: Dissolve the lyophilized powder in 270μL of water, then add 9.73mL of initial culture medium to prepare an expansion factor; total volume is 10mL. Store at -20°C or below.

Usage summary table

StepMedium
CoatingCK001-1A, 7.5mL coating solution
Activation Medium190mL initial culture medium + 10mL CK001-2A: Mix the two together for use
Expansion Medium180 mL initial culture medium + 10mL CK001-3A: Mix the two together for use

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