Manual

Product Number: PCK55

Shipping and Storage

Store at -20°C, with a shelf life of 12 months.

Components

Description

This product is a dedicated kit for one-step reverse transcription real-time fluorescence quantitative detection using the probe method. With extracted RNA as the template, reverse transcription and fluorescence quantitative detection are performed sequentially in the same reaction tube, ensuring simple operation while effectively preventing contamination and reducing pipetting errors. Based on high-efficiency reverse transcriptase, hot-start polymerase, and an optimized buffer system, it delivers excellent amplification performance for RNA templates with complex secondary structures and high GC content, making it highly suitable for detecting trace target genes such as RNA viruses. When using this product, simply add the template, primers, probes, ROX Reference Dye (used to correct fluorescence signal variations between wells, select based on the specific real-time PCR instrument), and water,

Adjust the working concentration to 1× to proceed with the reaction. It offers advantages such as rapid and simple operation, high sensitivity, strong specificity, and excellent stability, effectively minimizing human errors, saving PCR experimental time, and reducing contamination risks.

Application

Suitable for high-copy and low-copy gene detection; applicable to RNA templates with high GC content or complex secondary structures.