DNA Polymerase I, Large (Klenow) Fragment
2025-11-11
Recombinant Humanized Collagen, Type III Sterile Lyophilized Powder
2025-11-111st strand cDNA transcription Kit, 100prep
Product Number: PCK5802
Shipping and Storage
Low temperature transportation, stored at -20℃, with a shelf life of one year.
Component
| Component | 100T |
| MMLV RTase, 200U/µL | 100µL |
| RNase Inhibitor, 20U/µL | 100µL |
| 2×RT Buffer | 1mL |
| RNase free water | 1mL |
Description
This kit provides all the reagents required for synthesizing the first strand of cDNA. The principle is to efficiently synthesize full-length cDNA copies of mRNA using mutated Moroni murine leukemia virus reverse transcriptase (M-MuLV RT).
Features
- The use of mutated reverse transcriptase results in low endogenous RNase H activity.
- Can synthesize cDNA with a length of 9kb or more.
- Oligo dT and random primers can be used, which are provided in this kit.
- Total RNA, poly (A)+RNA, or special RNA can all be used as templates.
- Can be used for the synthesis of the second chain RT-PCR, Probe labeling, etc.
